I-390 is a freeway linking Rochester with the Southern Tier. The first section to open, between Wayland and Dansville, was originally part of NY 245. The expressway was considered to be part of NY 401 prior to being designated I-390 in 1973. While its route from I-590 to downtown Rochester was cancelled, it was re-routed onto the "Outer Loop" (formerly NY 47, which was to be entirely I-590) to I-490 (at one time, the I-390 designation was to reach NY 104, but this was denied by FHWA). It is extended by NY 390 to the Lake Ontario State Parkway.
| Mile | Northbound | Southbound | Notes |
|---|---|---|---|
| Steuben County | |||
| Speed Limit 65 | |||
| 0.0 |  |
 EastCorning |
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| 0.6 | West Jamestown |
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| 1.9 | Exit 1 To  Avoca |
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| 9.1 | Scenic Overlook | ||
| 10.9 | Exit 2 To Cohocton Naples |
 |
|
| 16.6 | Exit 3  Wayland |
End of former multiplex with NY 15; many signs still remain Begin former NY 245 overlap |
|
| Livingston County | Steuben County | ||
| 22.5 | Exit 4 Dansville |
Exit 4 Dansville Hornell |
|
| 24.2 | Exit 5 Dansville |
End former NY 245 overlap | |
| 33.1 | Exit 6 Mount Morris Letchworth State Park |
Exit 6 Sonyea |
|
| 38.1 | Geneseo Rest Area | Mount Morris Rest Area | |
| 38.9 | Exit 7  Geneseo |
Exit 7 Mount Morris Letchworth State Park |
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| 48.1 | Exit 8 Lakeville Conesus Lake |
Exit 8 Geneseo |
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| 51.6 | Exit 9 Avon |
Exit 9 Lakeville Conesus Lake |
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| 54.7 | Exit 10  Avon Lima |
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| Monroe County | Livingston County | ||
| 61.9 | Exit 11  Rush Scottsville |
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| 65.4 | Exit 12  Thruway Lehigh Station Road |
Exit 12B Thruway |
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| 65.9 | Exit 12A Lehigh Station Road |
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| 67.4 | Exit 13 Hylan Drive Mall |
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| 68.5 | Exit 14  East Henrietta Road Jefferson Road |
Exits 14A-B East Henrietta Road Jefferson Road |
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| Speed Limit 55 | Speed Limit 65 | ||
| 69.3 | Exit 15B Brighton-Henrietta Town Line Road |
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| 70.4 | Exit 15 NorthDowntown Rochester Irondequoit |
Exit 15A North Irondequoit |
End of Genesee Expressway |
| 71.2 | Exit 16B North East Henrietta Road Rochester |
||
| 71.2 | Exit 16A South East Henrietta Road West Henrietta Road |
Exit 16B East Henrietta Road |
Formerly exit 16 northbound University of Rochester Medical Center Monroe Community College |
| 71.7 | Exit 16A West Henrietta Road East River Road |
||
| 73.1 | Exit 17 Scottsville Road |
Former NY 47 | |
| 74.0 | Exit 18A Brooks Avenue East |
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| 74.0 | Exit 18B WestBrooks Avenue Airport |
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| 74.7 | Exit 19 Chili Avenue |
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| 76.1 | Exit 20A EastRochester |
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| 76.1 | Exit 20B West Buffalo |
Exits 20A-B Rochester Buffalo |
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| 76.6 | Exit 21 Lyell Avenue |
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| 77.6 | Exit 22 Lexington Avenue |
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| 78.5 | Exit 23 Ridgeway Avenue |
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| 79.6 | Exit 24A EastRidge Road |
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| 79.6 | Exit 24B West Ridge Road Greece |
Exit 24 Ridge Road Greece |
Former end of NY 47 |
| 81.3 | Exit 25 Vintage Lane |
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| 82.9 | Exit 26 Latta Road Commercial vehicles must exit |
Exit 26 Latta Road |
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| 84.0 | Exit 27A EastRochester |
||
| 84.0 | Exit 27B West Hamlin Beach |
 |
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| Speed Limit 55 | |||
| Monroe County | |||