NY 49 ends on the Utica-Rome Expressway, a freeway running from Rome to I-790 in Utica. It runs parallel to the Thruway for the last couple miles and NYSDOT is interested in extending the I-790 designation to Rome (but needs to eliminate the at-grade railroad crossing first). The initial section built was between NY 825 and NY 291 in response to high traffic volume and accidents on the old route. It was extended west to downtown Rome in the 80s and finally east to I-790 in 2003.
| Mile | Eastbound | Westbound | Notes |
|---|---|---|---|
| Oneida County | |||
| Speed Limit 40 | |||
| 49.5 |    |
   East Whitesboro Street Freeway Ends |
|
| Speed Limit 55 | Speed Limit 40 | ||
| 50.0 | Lawrence Street | Westbound traffic exiting merges onto eastbound NY 49 and exits there. | |
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| 50.4 | To  To   South  WestOneida |
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| 51.1 |  East  SouthWestmoreland |
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| 52.3 | East East Dominick Street Barneveld |
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| Railroad Crossing | |||
| Speed Limit 65 | Speed Limit 55 | ||
| 53.4 |  NorthGriffiss Technology Park Griffiss International Airport |
East North East Dominick Street Griffiss Technology Park Griffiss International Airport |
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| 58.8 | To  River Street Oriskany |
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| 60.8 |  Whitesboro Stittville |
End of freeway prior to extension to I-790 | |
| 61.9 |  Cavanaugh Road Whitesboro |
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| 63.9 |  Marcy-SUNY Parkway |
Formerly Edic Road, Marcy-SUNYIT Parkway | |
| 64.6 |  West  South  SouthDowntown Utica |
 West |
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| 64.6 | North North Poland Watertown |
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| 64.6 | To East East |
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| Speed Limit 65 | |||
| Oneida County | |||