Roads maintained by NYSDOT are further subdivided into 11 regions. These regions are drawn on county lines. Each region develops its own projects and policies (for example, Region 2 is famous for using boxed street names on signage). Each county also has its own code based on the region, usually following the alphabetical order of the county names, but exceptions exist when region boundaries change (such as Region 11 splitting off from Region 10). A map of the regions is available on the projects page of the New York State Department of Transportation website.
- Region 1
- Includes Albany (11), Essex (12), Greene (13), Rensselear (14), Saratoga (15), Schenectady (16), Warren (17), and Washington (18) Counties
- Region 2
- Includes Fulton (21), Hamilton (22), Herkimer (23), Madison (24), Montgomery (25), and Oneida (26) Counties
- Region 3
- Includes Cayuga (31), Cortland (32), Onondaga (33), Oswego (34), Seneca (35), and Tompkins (36) Counties
- Region 4
- Includes Genesee (41), Livingston (42), Monroe (43), Ontario (44), Orleans (45), Wyoming (46), and Wayne (47; formerly 37) Counties
- Region 5
- Includes Cattaruagus (51), Chautauqua (52), Erie (53), and Niagara (54) Counties
- Region 6
- Includes Allegany (61), Chemung (62), Schuyler (63), Steuben (64), and Yates (66) Counties
- Region 7
- Includes Clinton (71), Franklin (72), Jefferson (73), Lewis (74), and St. Lawrence (75) Counties
- Region 8
- Includes Columbia (81; was planned to be transferred to Region 1), Dutchess (82), Orange (83), Putnam (84), Rockland (85), Ulster (86), and Westchester (87) Counties
- Region 9
- Includes Broome (91), Chenango (92), Delaware (93), Ostego (94), Schoharie (95), Sullivan (96), and Tioga (97; formerly 65) Counties
- Region 10
- Includes Nassau (03) and Suffolk (07) Counties
- Region 11 (NYC)
- Includes Bronx (01 - The Bronx), Kings (02 - Brooklyn), New York (04 - Manhatten), Queens (05 - Queens), and Richmond (06 - Staten Island) Counties